Designer protein helps paralyzed mice walk again in breakthrough study
In a new study, German scientists have restored the ability to walk in mice that had been paralyzed after a complete spinal cord injury. The team created a “designer” signaling protein and injected it into the animals’ brains, stimulating their nerve cells to regenerate and share the recipe to make the protein.
Spinal cord injuries are among the most debilitating. Damaged nerve fibers (axons) may no longer be able to transmit signals between the brain and muscles, often resulting in paralysis to the lower limbs. Worse still, these axons cannot regenerate.
Previous studies have shown promise in restoring some limb function through spinal stimulation therapy, or by bypassing the injury site altogether. Other promising research in similar areas has involved using compounds that restore balance to the inhibitory/excitatory signals in the neurons of partially paralyzed mice, and transplanting regenerating nose nerve cells into the spines of injured dogs.
But in the new study, researchers from Ruhr-Universität Bochum (RUB) in Germany took a different path, aiming to repair the damaged axons with a protein they call hyper-interleukin-6 (hIL-6). As the name suggests, this is a synthetic version of a naturally occurring peptide, which has been tweaked to stimulate nerve cell regeneration.
“This is a so-called designer cytokine, which means it doesn’t occur like this in nature and has to be produced using genetic engineering,” says Dietmar Fischer, an author of the study.
In the study, the team tested hIL-6 on mice that had sustained a complete spinal cord crush, resulting in loss of function in both hind legs. They packaged the genetic instructions to produce hIL-6 into a common viral vehicle, and injected these into the sensorimotor cortex of the mice.
Not only did motor neurons near the site of injection begin to produce hIL-6 themselves, but they passed it along through axonal side branches to other neurons responsible for actions like walking. And sure enough, within a few weeks the mice regained function in their hind legs, even after just a single injection.
“Thus, gene therapy treatment of only a few nerve cells stimulated the axonal regeneration of various nerve cells in the brain and several motor tracts in the spinal cord simultaneously,” says Fischer. “Ultimately, this enabled the previously paralyzed animals that received this treatment to start walking after two to three weeks. This came as a great surprise to us at the beginning, as it had never been shown to be possible before after full paraplegia.”
As incredible as the results sound, it’s still obviously very early days for the research. The team says the next steps are to investigate in mice whether similar results can be achieved if the treatment is administered weeks after the injury, rather than immediately following. Hopefully in the long run the results may be applied to humans, but that wouldn’t be for many years yet, if ever.
The research was published in the journal Nature Communications.
Source: Ruhr-Universität Bochum